Structural basis for translation termination by archaeal RF1 and GTP-bound EF1{alpha} complex

Oct 9, 2012 Full story: Nucleic Acids Research

In eukaryotes and archaea, the class I and II RFs form a heterodimeric complex, and complete the overall translation termination process in a GTP-dependent manner.

Full Story
andrew j langham

London, UK

#1 Oct 10, 2012
When a stop codon appears at the ribosomal A site, the class I and II release factors (RFs) terminate translation. In eukaryotes and archaea, the class I and II RFs form a heterodimeric complex, and complete the overall translation termination process in a GTP-dependent manner. However, the structural mechanism of the translation termination by the class I and II RF complex remains unresolved. In archaea, archaeal elongation factor 1 alpha (aEF1α), a carrier GTPase for tRNA, acts as a class II RF by forming a heterodimeric complex with archaeal RF1 (aRF1). We report the crystal structure of the aRF1·aEF1α complex, the first active class I and II RF complex. This structure remarkably resembles the tRNA·EF–Tu complex, suggesting that aRF1 is efficiently delivered to the ribosomal A site, by mimicking tRNA. It provides insights into the mechanism that couples GTP hydrolysis by the class II RF to stop codon recognition and peptidyl-tRNA hydrolysis by the class I RF. We discuss the different mechanisms by which aEF1α recognizes aRF1 and aPelota, another aRF1-related protein and molecular evolution of the three functions of aEF1α. yes quite so we know protein p51 p53 p55 does exactly this' and correct correct codon sequence for transport RNA to and from the mitrochondria golgi complex!
andrew j langham

London, UK

#2 Oct 10, 2012
Hiv testosterone esters need quality research now! so avaccine can be produced to block rogue testosterone esters from transmitting HIV and the complex codon sequencing of the resultant mis-transcription mult-permatations of tat rev etc hiv amino chains and sud units thereof!

Tell me when this thread is updated:

Subscribe Now Add to my Tracker

Add your comments below

Characters left: 4000

Please note by submitting this form you acknowledge that you have read the Terms of Service and the comment you are posting is in compliance with such terms. Be polite. Inappropriate posts may be removed by the moderator. Send us your feedback.

Molecular Biology Discussions

Title Updated Last By Comments
Software for Biologics Data Management (Mar '14) Mar '14 Ocimum 1
Study Links BPA and Breast Cancer Tumor Growth (Mar '14) Mar '14 SANDY SMIITH 1
Loop Mediated Isothermal Amplification (LAMP) d... (Feb '14) Feb '14 Anonymous 1
Genes for endosomal NHE6 and NHE9 are misregula... (Feb '14) Feb '14 Another Duh 25
Nucleocapsid protein structures from orthobunya... (Jun '13) Nov '13 Langham virus Res... 3
Making Rubber from Dandelion Juice (Oct '13) Oct '13 phytorem 1
cDNA synthesis (Sep '13) Sep '13 Gfritz293 1
•••
Enter and win $5000
•••

Molecular Biology People Search

Addresses and phone numbers for FREE

•••