Molarity of Solutions
Posted in the Biochemistry Forum
#1 Jan 11, 2012
Just having an issue with making up a solution and don't trust myself that i am doing it properly!
My protocol says that i need to prepare 0.05M Tris HCL buffer PH 7.4, containing 1.0M NACL, 20mM EDTA, 1mM PMSF.
I need to make 3ml in total.
I have 1M stock solution of Tris HCL buffer PH 7.4, and EDTA, NACL, and PMSF in solid form.
I am thinking that I probably do not need the NACL as its purpose was probably to adjust the PH to the desired level but as mine is already at 7.4 i might not need it.
Does anybody know the right quantities to make up this solution or can offer any advice in this area i would greatly appreciate it.
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