Molarity of Solutions

Limerick, Ireland

#1 Jan 11, 2012
Just having an issue with making up a solution and don't trust myself that i am doing it properly!

My protocol says that i need to prepare 0.05M Tris HCL buffer PH 7.4, containing 1.0M NACL, 20mM EDTA, 1mM PMSF.

I need to make 3ml in total.

I have 1M stock solution of Tris HCL buffer PH 7.4, and EDTA, NACL, and PMSF in solid form.

I am thinking that I probably do not need the NACL as its purpose was probably to adjust the PH to the desired level but as mine is already at 7.4 i might not need it.

Does anybody know the right quantities to make up this solution or can offer any advice in this area i would greatly appreciate it.



Tell me when this thread is updated:

Subscribe Now Add to my Tracker

Add your comments below

Characters left: 4000

Please note by submitting this form you acknowledge that you have read the Terms of Service and the comment you are posting is in compliance with such terms. Be polite. Inappropriate posts may be removed by the moderator. Send us your feedback.

Biochemistry Discussions

Title Updated Last By Comments
News Miniature droplets could solve an origin-of-lif... Oct '17 Qedlin Saltum 1
News University of Maryland researcher's published a... (Feb '17) Feb '17 noobieR 1
News Poop, there it is: New test for exotic civet co... (Sep '13) Feb '17 Fancy Phart 4
News Dr. Hays Young, Dna Analyst and Technical Leade... (Feb '17) Feb '17 Wildbird 1
Will this have ruined my health? (Feb '17) Feb '17 Njhuckman 1
News Association of rare missense variants in the se... (Dec '16) Dec '16 Stephany McDowell 1
News Researchers uncover a new mechanistic understan... (Oct '16) Oct '16 Stephany McDowell 1
More from around the web