Hydrolysis of proteins by sodium hydroxide
Posted in the Biochemistry Forum
#1 Mar 9, 2012
Need some serious help from those that understand hydrolysis of proteins. Have a protocol that requires treating cell with 3 M NaOH to denature DNA. I want to then perform immunocytochemistry but cannot because strong base causes hydrolysis, I think if I am reading my lit right. Is this irreversible? Is there anything that I could do to protect the proteins? Any suggestions would be greet, wondering if include salts or good neutralization buffer if could salvage the procedure.
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