Hydrolysis of proteins by sodium hydr...

Hydrolysis of proteins by sodium hydroxide

Posted in the Biochemistry Forum

Larispoli

Franklin, TN

#1 Mar 9, 2012
Need some serious help from those that understand hydrolysis of proteins. Have a protocol that requires treating cell with 3 M NaOH to denature DNA. I want to then perform immunocytochemistry but cannot because strong base causes hydrolysis, I think if I am reading my lit right. Is this irreversible? Is there anything that I could do to protect the proteins? Any suggestions would be greet, wondering if include salts or good neutralization buffer if could salvage the procedure.

Tell me when this thread is updated:

Subscribe Now Add to my Tracker

Add your comments below

Characters left: 4000

Please note by submitting this form you acknowledge that you have read the Terms of Service and the comment you are posting is in compliance with such terms. Be polite. Inappropriate posts may be removed by the moderator. Send us your feedback.

Biochemistry Discussions

Title Updated Last By Comments
News University of Maryland researcher's published a... Feb '17 noobieR 1
News Poop, there it is: New test for exotic civet co... (Sep '13) Feb '17 Fancy Phart 4
News Dr. Hays Young, Dna Analyst and Technical Leade... Feb '17 Wildbird 1
Will this have ruined my health? Feb '17 Njhuckman 1
News Association of rare missense variants in the se... (Dec '16) Dec '16 Stephany McDowell 1
News Researchers uncover a new mechanistic understan... (Oct '16) Oct '16 Stephany McDowell 1
News Why the Air Force Wants to See Pilots' Sweat (Sep '16) Sep '16 Stephany McDowell 3
More from around the web