Earlier studies have concluded EM-35 had origin in Horn of Africa/Northern Africa. However, National Geography recently come up with different finding, the haplotype originated in the Middle East before 20kya years.The E1b1b1-M35 lineages in some Pakistani Pashtun were previously traced to a Greek origi brought by Alexander's invasions . However RM network of E1b1b1-M35 found that Afghanistan's lineages are correlated with Midd Easterners and Iranians but not with population from the Balkans.Eee
"The man who gave rise to marker M35 was born around 20,000 years ago in the Middle East. His descendants were among the first farmers and helped spread agriculture from the Middle East into the Mediterranean region."
This will lead to a conclusion that most of modern Horners are Middle Eastern in origin, at least paternally. However, the origin of its ancesster EM-215 is still in the Horn, and all this tells you one important message, Horners and ancient Middle Easterns are genetically inseparable.